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mycobacterial expression vector pyubclic gfp  (Addgene inc)


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    Addgene inc mycobacterial expression vector pyubclic gfp
    Mycobacterial Expression Vector Pyubclic Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyub28b+vector/pYUB28b+(Plasmid+%2337277)/pm35750296-192-1-13
    Average 90 stars, based on 2 article reviews
    mycobacterial expression vector pyubclic gfp - by Bioz Stars, 2026-10
    90/100 stars

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    Expressing:

    Article Title: High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP.
    Article Snippet: All enzymes were purchased from ThermoFisher scientific, unless stated otherwise. .. The Mycobacterial expression vector pYUBcLIC-GFP was built on the backbone of pYUB28b [48] (Addgene plasmid # 37277; http://n2t. net/addgene:37277; RRID:Addgene_37277), which was linearized by double digestion with NcoI and HindIII, and dephosphorylated using FastAP thermosensitive alkaline phosphatase. .. The digested vector was purified from 0.5% (w/v) agarose gel (E.Z.N.A.

    Plasmid Preparation:

    Article Title: High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP.
    Article Snippet: All enzymes were purchased from ThermoFisher scientific, unless stated otherwise. .. The Mycobacterial expression vector pYUBcLIC-GFP was built on the backbone of pYUB28b [48] (Addgene plasmid # 37277; http://n2t. net/addgene:37277; RRID:Addgene_37277), which was linearized by double digestion with NcoI and HindIII, and dephosphorylated using FastAP thermosensitive alkaline phosphatase. .. The digested vector was purified from 0.5% (w/v) agarose gel (E.Z.N.A.

    Modification:

    Article Title: Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
    Article Snippet: The codon-optimised gene was synthesised and cloned into a pYUB28b-TEV vector by Bio Basic Inc. .. The vector pYUB28b-TEV is modified from pYUB28b (Addgene #37277) that was provided by Dr. Ghader Bashiri from The University of Auckland. ..



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    Oligonucleotide primers used in the amplification of the protein coding sequences in this study.

    Journal: PLoS ONE

    Article Title: Metabolic Engineering of Cofactor F 420 Production in Mycobacterium smegmatis

    doi: 10.1371/journal.pone.0015803

    Figure Lengend Snippet: Oligonucleotide primers used in the amplification of the protein coding sequences in this study.

    Article Snippet: The nucleotide sequences for pYUB28b and pYUBDuet vectors have been deposited in the National Centre for Biotechnology Information (NCBI) under GenBank HQ247814 and HQ247815 accession numbers, respectively.

    Techniques: Amplification